
Presenter:
Jack T. Leonard, Ph.D.
Vice President of Technology Commercialization, febit inc.
Abstract:
Massively parallel DNA sequencing technologies, partnered with sequence capture methodologies, have accelerated the investigation of specific genomic regions while reducing the time, cost, and effort needed to interrogate multiple loci. After automated sequence capture with HybSelect, targeted genomic sequencing was carried out on the SOLiD System. We demonstrated that high levels of multiplexing are achievable by combining HybSelect parallel sample processing capabilities with the new bar-coding reagents for SOLiD from Life Technologies. Up to 320 bar-coded samples can be sequenced every 6 days using a SOLiD Sequencing System with a febit Geniom RT Analyzer. The sample throughput will continue to increase with the introduction of additional bar-codes.
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