Transcriptome sequencing for RNA discovery, profiling and quantitation

As an official ABI service provider febit’s Genomic Services offer sequencing services for discovery studies both on the genomic and the transcriptional level. Finding new DNA or RNA biomarkers is key to progress in this important research field.

febit’s Genomic Services offer sequencing-based applications for discovering, identifying and profiling of coding and non-coding RNAs. This includes

• Whole transcriptome sequencing
• Sequencing-based RNA discovery and profiling
• Identification of novel coding and non-coding RNAs in any species
• Alternative splicing
• Complete view and accurate quantitation of the transcriptome
• Identification of low-abundance RNAs and transcript variant

RNA sequencing has emerged as a powerful technology for exon-level expression analysis. By mapping millions of RNA sequencing reads to individual transcripts and exons, it is possible to estimate the overall abundance of the mRNA transcripts as well as the splicing levels of individual exons. Cells use alternative splicing to create alternative RNA transcripts from the same stretch of DNA, and RNA sequencing is the tool of choice to directly examine this alternative splicing. More over, RNA sequencing can be used to investigate both gene expression and alternative splicing in the same data set.

Unlike the genome, the transcriptome dynamically changes in response to the environment or to intrinsic processes. Therefore, RNA sequencing studies aim at identifying transcriptome sequences not only for different cell types but also for different physiological conditions.
The use of NGS technologies for RNA analyses is particularly attractive for the quantitative analysis of transcript expression levels. Studies published in this field have shown that RNA sequencing data are highly quantitative and give reliable measurements of transcript levels in one or more conditions.

SOLiD Sequencer

The Applied Biosystems SOLiD™ 3 System is a highly accurate next-generation sequencing platform that enables massively parallel sequencing of clonally amplified DNA fragments linked to beads. This sequencing methodology is based on sequential ligation with dye-labeled oligonucleotides. The ultra high throughput, coupled with broad application flexibility, provides a unique system with greater than 99.94% basecalling accuracy. This is due to the robust ligation-based sequencing chemistry with the 2 Base Encoding system. 2 Base Encoding enables unique error checking capability, providing higher confidence in each call. Unmatched accuracy complemented by a broad instrument scalability make the SOLiD 3 System a revolutionary, robust genetic analysis platform.