Gene Expression Profiling - Overview

RNA expression profiling

The transcriptome represents the collection of all RNAs produced in a cell or tissue at a defined time in development. Within biological systems it provides the critical link in the flow of information between genes and disease.

Discover the transcriptome

febit’s Genomic Services offer the whole range of technology to explore the transcriptome

Take advantage of our cutting-edge technologies to perform comprehensive studies:

  • RNA discovery with NGS
  • Expression profiling for genes of interest or non-coding RNAs on validated microarrays
  • Validation of results using qRT-PCR

Your study is completed with an expert bioinformatics report that provides you with the entire set of results and a full-scale biostatistical analysis.

Combine miRNA and target gene analysis to identify genetic networks

Take your miRNA study to the next level: For a comprehensive analysis of your sample, perform expression profiling of predicted target genes and identify affected pathways

  • Combined analysis of miRNAs and their target mRNAs
  • Target gene prediction by expert bioinformatics
  • Pathway specific gene expression profiling
  • Comprehensive statistical analysis of regulated networks

Get the most out of your sample and utilize the versatility of the Geniom RT Analyzer: Using the same platform for analyzing both coding and non-coding RNAs guarantees reliable data and minimized experimental bias for a complete view on your sample.

Prediction and analysis of target genes

Since each miRNA can regulate one to several messenger RNA transcripts, and conversely one mRNA can be regulated by one to several miRNAs, it is of greatest interest to researchers to identify those connecting networks. febit’s bioinformatics service applies established computational methods for target gene prediction. Geniom Biochips and the Geniom RT Analyzer instrument are subsequently employed to perform gene expression profiling based on the identified gene sequences. Pathway specific arrays enable the identification of regulated genetic networks.

febit offers NGS, array and qRT-PCR technologies for your transcriptomics studies

Transcription and maturation of RNAs are tightly controlled processes shaping complex gene expression networks that ultimately drive biological processes. An in-depth understanding of these principles and mechanisms is important to better understand complex diseases such as cancer. Microarrays allow the simultaneous monitoring of expression levels of all annotated genes in any given cell population. Additionally, with the increasing realization that RNAs transcribed from non-coding portions of the genome are playing fundamental roles, genome-wide approaches provide valuable insight into this aspect of transcriptomes, too. Bringing RNA analysis to an even higher level, with the use of next generation sequencers transcriptome maps can be obtained at the best possible resolution, potentially revolutionizing the field of transcriptomics.

With febit’s Genomic Services you can take advantage of the full range of established technologies to perform comprehensive studies. Discover RNAs with NGS, define expression profiles of genes of interest or non-coding RNAs on validated microarrays, and validate your results using qRT-PCR. Completing your study is the expert bioinformatics report that provides you with the entire set of results and a full-scale biostatistical analysis.

RNA expression profiling plays a fundamental role in clinical research as well as in other research fields such as developmental biology, stem cell research, virology or microbiology. Substantial knowledge is being generated looking into transcriptional processes and biological mechanisms from many different organisms.