Geniom RT Analyzer
automated microarray processing and analysis
The Geniom® RT Analyzer is designed for the automated processing of microarray experiments. It uses microfluidic Geniom Biochips for a wide range of applications.
The instrument at a glance:
- all-in-one instrument for sample loading, hybridization, washing, staining, and detection of biochips
- automation with easy and streamlined protocols
- reproducibility due to precise fluidic control and automation
- microfluidic biochips enable precise fluidic control, automation and flexible probe content
All-in-one instrument
The Geniom RT Analyzer is a hybridization oven, a wash station, a shaker and a scanner in one instrument. Sample loading, hybridization, washing, precise temperature control, staining and detection are fully automated. There is no need for many instruments. Also the experimental workflow is much easier when you have several instruments integrated into one instrument (exact timing, exact pipetting control, minimal hands-on time). The software for the Geniom RT Analyzer is intuitive and easy to use. Users can either run experiments with pre-installed programs from febit or write new programs with ease. The Geniom RT Analyzer works with a precise temperature control for optimal hybridization. A pressurized system allows the automated sample movement through the microchannels of the biochips with accurate timing. This automated back and forth moving of the sample during the hybridization increases the sensitivity and stringency while reducing hybridization time
Automation
Through the fluidic control within the microfluidic biochips, automation is possible for the whole hybridization experiment. Consequently very short hands-on time is needed for you doing a hybridization experiment. Only buffer, reagent tubes and sample tubes have to be positioned in the right place. Sample loading and hybridization, washing, shaking and detection are done automatically. The Geniom RT Analyzer software has program steps for loading, hybridization, washing and shaking. For many experiments febit provides software profiles with these program steps for the Geniom RT Analyzer. The software is also intuitive and enables you to write your own experiment profiles with ease. You can drag and drop the program steps and can change the variables like time and temperature during this step. A CCD camera can be programmed to generate detection pictures. You import these pictures in a software tool for analyzing the microarray experiment. The microarray experiment with the Geniom RT Analyzer needs only short hands-on time. This increases your productivity, because you have more time for other research work.
Reproducibility
Because of the automation of the microarray experiment with the Geniom RT Analyzer it is very reproducible. The timing, the pipetting volumes and the incubation temperatures in each experiment are exactly the same. This is enabled by the microfluidic Geniom Biochip from febit.
| reproducibility and sensitivity: | active motion hybridization: |
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the unique closed microfluidic system
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| multi-purpose: | small sample size: |
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| ease of use: | flexibility: |
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| throughput: | |
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| Sample Handling | |
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| Volume per sample | max. 40 μl |
| Sample throughput | typically 8 -16 samples per day depending on application |
| Sample injection | manual |
| Processing times | hybridization 1 - 16 hours depending on application |
| Detection | |
| Detection | Phycoerythrin & Cy3 fluorescence detection, additional dyes please inquire |
| CCD camera | 8 megapixel CCD camera |
| CCD camera resolution | 6 micrometer |
| Dynamic range | 3 - 4 orders of magnitude |
| Excitation wavelength (Emax) | 545 nm |
| Time for picture acquisition | 0.2 - 5 sec. |
| Dynamic detection | yes |
| Feature extraction | automated feature extraction |
| Software | |
| Instrument software | Geniom Software for instrument control
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| Raw data export | Tab delimited format ensures compatibility to major bioinformatic software packages for high level data analysis |
| Data analysis | Luma Software Package for Array Design and Data Analysis |
| Phyiscal Specifications | |
| Light source | UHP Mercury lamp |
| Power | 240 V / 110 V, 50 - 60Hz, 150 W |
| Size dimensions | 55.7 cm × 90.7 cm × 66.5 cm |
| Weight | 110 kg |
| Network connection | Ethernet / USB |
| Maintenance | Remote Access Option |
| Geniom Biochips | |
| Chip format | microfluidic Geniom Biochips |
| Arrays per biochip | 8 |
| Features per array | currently up to 15,000 |
| Features per biochip | currently up to 120,000 |
| Capture probe design | depending on application, available standards:
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Software controlled automation power
Operation of the Geniom RT Analyzer is controlled by the intuitive instrument control software. The software provides predefined protocols for all standard and common applications. Development of additional protocols is fast and efficient. The instrument status is displayed in real-time through status messages, including the current temperature or processed array, all while the machine is performing each individual programmed step.
Geniom RT Analyzer Control Software key features:
- Standard protocols
- Easy customization
- Real-time process monitoring
In addition to instrument control software, the Geniom Wizard Software tools allow microarray imaging, feature recognition and quantification. The software supports tab delimited data export for seamless compatibility with most bioinformatics and data analysis packages.

