Geniom RT Analyzer

automated microarray processing and analysis

The Geniom® RT Analyzer is designed for the automated processing of microarray experiments. It uses microfluidic Geniom Biochips for a wide range of applications.

The instrument at a glance:

  • Geniom RT Analyzer with workstation
    Geniom RT Analyzer with workstation
  • all-in-one instrument for sample loading, hybridization, washing, staining, and detection of biochips
  • automation with easy and streamlined protocols
  • reproducibility due to precise fluidic control and automation
  • microfluidic biochips enable precise fluidic control, automation and flexible probe content

All-in-one instrument

The Geniom RT Analyzer is a hybridization oven, a wash station, a shaker and a scanner in one instrument. Sample loading, hybridization, washing, precise temperature control, staining and detection are fully automated. There is no need for many instruments. Also the experimental workflow is much easier when you have several instruments integrated into one instrument (exact timing, exact pipetting control, minimal hands-on time). The software for the Geniom RT Analyzer is intuitive and easy to use. Users can either run experiments with pre-installed programs from febit or write new programs with ease. The Geniom RT Analyzer works with a precise temperature control for optimal hybridization. A pressurized system allows the automated sample movement through the microchannels of the biochips with accurate timing. This automated back and forth moving of the sample during the hybridization increases the sensitivity and stringency while reducing hybridization time

Automation

Through the fluidic control within the microfluidic biochips, automation is possible for the whole hybridization experiment. Consequently very short hands-on time is needed for you doing a hybridization experiment. Only buffer, reagent tubes and sample tubes have to be positioned in the right place. Sample loading and hybridization, washing, shaking and detection are done automatically. The Geniom RT Analyzer software has program steps for loading, hybridization, washing and shaking. For many experiments febit provides software profiles with these program steps for the Geniom RT Analyzer. The software is also intuitive and enables you to write your own experiment profiles with ease. You can drag and drop the program steps and can change the variables like time and temperature during this step. A CCD camera can be programmed to generate detection pictures. You import these pictures in a software tool for analyzing the microarray experiment. The microarray experiment with the Geniom RT Analyzer needs only short hands-on time. This increases your productivity, because you have more time for other research work.

Reproducibility

Because of the automation of the microarray experiment with the Geniom RT Analyzer it is very reproducible. The timing, the pipetting volumes and the incubation temperatures in each experiment are exactly the same. This is enabled by the microfluidic Geniom Biochip from febit.

 

reproducibility and sensitivity: active motion hybridization:
the unique closed microfluidic system
  • enables reliable and reproducible results
  • eliminates handling artifacts such as bubbles, and protects microarrays from environmental elements such as ozone and ambient light
  • eliminates cross-contamination by providing physical separation of 8 microarray compartments
  • increases sensitivity and stringency while reducing hybridization time by automated sample movement during hybridization
  • pressurized system drives set amounts of samples through the microchannels in a precisely timed and accurate manner
  • capillary forces generated within each microchannel enhance the probability of samples finding their target probe
multi-purpose: small sample size:
  • one instrument for a wide range of applications
  • automated liquid handling allows precise and efficient processing of small sample sizes
ease of use: flexibility:
  • completely hands-off experience: biochip loading, docking with the microfluidic system, microarray optical calibration, hybridization, processing and detection are fully automated; sample loading and connection of buffer bottles are the only manual steps.
  • intuitive experimental design and setup supported by the Geniom Software which guides the user through all experimental steps.
  • offers a wide range of applications.
  • provides various automated protocols for standard microarray experiments and supports generation of customized protocols as well.
  • enables customization due to the complete control of automated sample movement, liquid handling, time and temperature; thus allowing introduction of various buffers, dyes and even enzymes at will.
throughput:  
  • enhances throughput via parallel processing of 8 physically-separated arrays
  • simultaneous analysis of up to 8 independent samples.
  • reduces overall processing time via a high degree of automation and active motion hybridization
 
Sample Handling  
Volume per sample max. 40 μl
Sample throughput typically 8 -16 samples per day depending on application
Sample injection manual
Processing times hybridization 1 - 16 hours depending on application
Detection  
Detection Phycoerythrin & Cy3 fluorescence detection, additional dyes please inquire
CCD camera 8 megapixel CCD camera
CCD camera resolution 6 micrometer
Dynamic range 3 - 4 orders of magnitude
Excitation wavelength (Emax) 545 nm
Time for picture acquisition 0.2 - 5 sec.
Dynamic detection yes
Feature extraction automated feature extraction
Software  
Instrument software Geniom Software for instrument control
  • Standard protocols
  • Easy customization
  • Real-time process monitoring
Raw data export Tab delimited format ensures compatibility to major bioinformatic software packages for high level data analysis
Data analysis Luma Software Package for Array Design and Data Analysis
Phyiscal Specifications  
Light source UHP Mercury lamp
Power 240 V / 110 V, 50 - 60Hz, 150 W
Size dimensions 55.7 cm × 90.7 cm × 66.5 cm
Weight 110 kg
Network connection Ethernet / USB
Maintenance Remote Access Option
Geniom Biochips  
Chip format microfluidic Geniom Biochips
Arrays per biochip 8
Features per array currently up to 15,000
Features per biochip currently up to 120,000
Capture probe design depending on application, available standards:
  • shortmer designs up to 30mers
  • longmer designs up to 60mers
  • inversed oligonucleotides with free 3' end for enzymatic reactions available
Software controlled automation power

Operation of the Geniom RT Analyzer is controlled by the intuitive instrument control software. The software provides predefined protocols for all standard and common applications. Development of additional protocols is fast and efficient. The instrument status is displayed in real-time through status messages, including the current temperature or processed array, all while the machine is performing each individual programmed step.

Geniom RT Analyzer Control Software key features:
  • Standard protocols
  • Easy customization
  • Real-time process monitoring

In addition to instrument control software, the Geniom Wizard Software tools allow microarray imaging, feature recognition and quantification. The software supports tab delimited data export for seamless compatibility with most bioinformatics and data analysis packages.

Schematic RT Analyzer Workflow

1. Select your Geniom Biochip 2. Prepare your samples 3. System set-up 4. Insert the Geniom Biochip 5. Add your samples 6. Run the experiment and readout the results