DNA-Synthesis with Geniom Technology

enabling standard and custom biochips

DNA-Synthesis takes place in the Geniom® One Instrument, a compact benchtop device that utilizes microfluidics for light-activated oligonucleotide synthesis and QC. This enables dynamic, effective and flexible handling of all reagents and processes required for multiplex DNA synthesis.

You can take advantage of febit’s synthesis technology with the following options:

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  Genomic Services

  febit’s offers comprehensive Genomic Services for microRNA Expression Profiling, Gene Expression Profiling and other applications.

  

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  Geniom Biochips

  The catalog and custom biochips can be ordered from febit and processed with the automated Geniom RT Analyzer. There are more than 500 Catolog Geniom Biochips available.

  

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  Geniom Microarray Facility

  This solution allows to synthesize Geniom Biochips in the customers lab and automatically process and analyze them afterwards.

   

Light-activated in situ oligonucleotide synthesis with Geniom One

A digital micromirror device (DMD) in combination with a high-pressure mercury lamp (350-750 nm) carries out light-activated, spatially resolved deprotection of photolabile groups immobilized inside the Geniom Biochip microchannels. Deflection of single micromirrors is addressed by software control.

When amidite DNA building blocks are added, coupling takes place exclusively at those spots which have been deprotected. Repeated cycles of photodeprotection and amidite coupling result in oligonucleotide chain elongation with ologonucleotide sequences specific for each spot.

Geniom’s extraordinary flexibility enables tailored oligonucleotide synthesis: any oligonucleotide sequence with any length (10-60mers) even within one array (compensate for different hybridization temperatures of probes), any probe parameter setting (optimized AT/GC content or melting point), and any array design is possible.

The current Geniom synthesis sets support 3’ to 5’ and 5’ to 3’ synthesis of 10- to 30mer oligonucleotides. Longmer sets for standard synthesis of up to 60mers are also available.

Geniom One automatically

• maps the array design on the biochip
• links the photolabile-protected spacer to the glass surface
• deprotects the spacer in selected spots by the micromirror-directed light beam
• adds protected amidite DNA building blocks that will couple only on deprotected spots
• builds up specific DNA-sequences by repeated cycles of selective light-activated deprotection and coupling of amidites
• enables sample hybridization by a final activation step