Consecutive Signal Enhancement

A protocol to enhance the sensitivity of experiments with limited sample material or weak hybridization signals.

How does it work?

Geniom® Consecutive Signal Enhancement (CSE) protocol is an accurate way to enhance the sensitivity of a microarray experiment on demand. Employing a fully automated 2 step protocol, the Geniom System quickly performs an antibody mediated signal amplification step leading to a second dataset with an enhanced sensitivity level derived from the same microarray.

The microfluidic system of the Geniom Biochip makes it possible to easily add the required solutions and let the instrument automatically perform the incubation and washing steps. It only takes a few mouseclicks to send the additional solutions through the microchannels and get dramatically improved results. The microfluidic reaction carrier ensures consistent reaction conditions within the microchannels as well as high quality oligonucleotide probes, guaranteeing reproducible and reliable data.

Why CSE?

The accuracy of genetic data depends on the experiment itself and further image processing. However, it has been shown that certain microarray applications require enhanced detection sensitivity due to limited sample material or weak hybridization signals.

How can you use CSE?

The CSE protocol works with the Geniom Microarray Facility (read more) and the Geniom RT Analyzer (read more).

Reliable data with CSE

Data integrity and reproducibility

Datasets of step 1 and step 2 of the CSE-protocol are plotted against each other, showing the high concordance of results obtained before and after sensitivity enhancement of the same microarray experiment.

High signal amplification, low noise amplification

 

Geniom empowered signal enhancement via CSE
The overall amplification factor of signal intensity is > 4
The background amplification factor is ~ 2
Signal to noise ratio increases by factor 5

Scatterplot highlighting the data integrity and reproducibility of the results. Datasets of step 1 and step 2 of the CSE-protocol are plotted against each other, showing the high concordance of results obtained before and after sensitivity enhancement of the same microarray experiment.

Step 1 of CSE

SAPE labeling of the biotinylated target generates a first dataset. In a standard experimental procedure, the biotinylated target, which is hybridized to the oligonucleotide probes on the microarray, is stained with streptavidin r-phycoerythrin conjugate (SAPE) in a fully automated manner, and a first dataset is generated.

Step 2 of CSE

Geniom Consecutive Signal Enhancement (CSE)-protocol. Fully automated, simultaneous incubation of the same microarray with a mixture of a biotinylated antibody and the SAPE conjugate for signal amplification.