Dynamic Detection

A technique to run multiple detections at different stages of your experiment providing a key aspect in quality control.

How does it work?

In contrast to many microarray platforms, the Geniom® Technology provides the user with a unique feature that allows specific measurements and quality control via real-time monitoring on the chip: the dynamic detection of hybridization.

Dynamic detection, the generation of CCD-images at defined time points. Digital imaging within the scope of a specific temperature program allows for precise monitoring of every single reaction on a microarray, the determination of melting points as well as the quantification of dsDNA during the annealing phase. Generation of melting curves to ensure product specificity is essential, particularly for enzyme-based applications on microarrays. The selective and sensitive detection of the fluorophores gives the user full control of his experiments. High-resolution in terms of quality and intervals of the acquired pictures allows user-defined, synchronized checks of every individual reaction in the microchannels of the Geniom biochip.

Why Dynamic Detection?

Dynamic Detection represents an ideal selection tool for the determination and optimization of the right and specific probes, since probe-sample behavior is not just predicted, but can be checked at any temperature during the experiment. Documentation and analysis of the dynamic hybridization enables a specific selection process to ensure ideal probe composition for every analyzed sequence.

Applications like SNP typing or Dynamic Allele-Specific Hybridization (DASH) are supported by this additional feature, since synchronized monitoring during the heating process allows high-level sequence determination at a single base resolution.

Another application is the use of febit’s 3’-inversed synthesis set for reverse (3’-up) synthesis of DNA oligonucleotides in the biochip. The emerging probes present free 3’-ends accessible to enzymes like nucleic acid polymerases, providing a broad spectrum of new enzyme based applications for DNA microarrays, like PCR-on-chip or primer extension reactions. These applications benefit from the dynamic detection, since the technology offers real-time control over temperature and fluorescence monitoring to check for amplification rate and product specificity.

How can you use Dynamic Detection?

The Dynamic Detection technique works with the Geniom Microarray Facility (read more) and the Geniom RT Analyzer (read more).

Reliable data with Dynamic Detection